X-Cite 120 Series Datasheet by Excelitas Technologies

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X-Cite® Guide
X-Cite® 120 Series Maintenance
& Troubleshooting Guide
1. Introduction
This guide contains installation, maintenance and troubleshooting tips to help optimize the lifetime and
performance of X-Cite® 120 series and components. This information is intended to supplement the detailed
installation and operating instructions found in the X-Cite® User Manuals.
over time and require maintenance or replacement. Note that the lifetime information on components is for typical
or average conditions. Depending on the imaging application, more frequent replacement may be required—for
output spectrum.
2. Liquid Light Guides
2.1 Proper handling and installation of liquid light guides
Liquid light guides have a typical useful life of 4000 hours of operation when handled properly and installed in a
well maintained X-Cite® 120. The formation of bubbles is one of the most common reasons for a light guide to
degrade prematurely and result in a sudden reduction in illumination intensity. Bubbles can form without warning,
stress to the light guide. Below are some simple tips to avoid overheating and stressing the light guide.
Always fully insert the light guide into the X-Cite® unit (i.e. up to the white line); 1.
this ensures contact with a heat sink to conduct heat away from the light guide.
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X-Cite® Guide EKCELITAS nnnnnnnnnnnnn
Always allow adequate clearance at the rear of the X-Cite® unit and microscope to 2.
prevent excessive bending and/or crushing of the light guide against walls. Minimum
bend radius to prevent immediate damage to the LLG is 40mm, however, a bend
radius of at least 75mm is recommended for a light guide while “in use. Sharper
bends can cause heat to build up and cause problems longer term.
Always place the X-Cite® unit close enough to the microscope so that there is some 3.
slack in the light guide and no sharp bends.
Never leave an endcap on the output end of the light guide when the other end is 4.
connected to the X-Cite® unit; if the unit is turned on in this condition, the cap will
overheat, melt and/or permanently discolour the quartz end of the light guide.
Do not expose the light guide to extreme temperatures (above 35°C, below -5°C) for 5.
extended periods of time during use, transport or storage; this may cause
degradation of the seals and allow air bubbles to form in the liquid.
2.2 What does a bubble look like?
Depending on the size and location in the light guide, a bubble may or may not be obvious. To check for bubbles:
Disconnect the LLG from the X-Cite® and microscope adapter. 1.
Hold one end towards a bright window or overhead room light - DO NOT use an 2.
X-Cite® or any other focused light source for this test!
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Look at the quartz at the other end of the LLG 3.
Bubble-free: quartz end will appear as a bright, solid circle; you may also be a.
able to see a thin circular outline at the quartz/liquid interface.
Bubbles at/near the quartz end: appear as dark spots, as small as 0.5mm in b.
will appear as dark shadows.
In extreme cases, where the bubble is blocking the entire diameter of the d.
light guide, no light will come through, even when pointing the distal end at a
light source.
2.3 Can a bubbled light guide recover?
Yes, light guides with small bubbles can sometimes recover. Disconnect the light guide from the X-Cite® unit, and
bubble when it is small.
2.4 When should light guides be replaced?
It is usually time to replace a light guide when:
Illumination is low and replacing the lamp does not improve brightness
the light)
A section of the light guide becomes noticeably warmer than the rest of the guide (a
bubble is blocking transmission of light, forcing the light guide to absorb the energy)
It is 2-3 years old OR has been in use for 4000-6000 hours (2-3 lamp changes), when
handled properly in a well maintained unit.
Appearance and % output of light guides with
bubbles relative to an LLG without bubbles (100%).
100% 11.3% 5.2%
X-Cite® Guide
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3.1 Performance and lifetime
The lamp output spectra and typical output levels over lifetime are shown below.
3. Lamps
the initial output for the remainder of the lamp life. If the lower power regions of the output spectra are being used,
it may be desirable to replace lamps more frequently to maximize signal levels.
Elapsed Time (Hours)
0 200 400 600 800 1000 1200 1400 1600 1800 2000
Relative Intensity (%)
Typical Lamp Intensity Over Lifetime for X-Cite® 120Q
Wavelength (nm)
Relative Intensity (arbitrary units)
300 400 500 600 700
X-Cite 120Q
Relative Output X-Cite® 120Q vs. HBO 100
X-Cite® Guide
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3.2 General tips to help maximize lamp lifetime
5 hours, therefore it is recommended to avoid shutting down the unit during lunch or
short breaks or between users, if being used in a multi-user lab
Avoid attempting to strike a hot lamp (note: the X-Cite®’s Intelli-Lamp® will prevent this) 3.
Avoid unnecessary movement and jarring of the lamp, especially when it is in 4.
operation or hot
When changing a lamp: 5.
Handle the lamp only by the ceramic areas a.
Never touch the glass envelope of the bulb (inner stem), the inner surface, or b.
alcohol. Skin oils can etch the glass and cause premature bulb failure
Wear cotton gloves or powder free latex/nitrile gloves when handling any lamp c.
can reduce lamp lifetime to approximately 25% of what is normally expected.
X-Cite® Guide
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4. Air Vents and Filters
X-Cite® units have several air vents that are an integral part of the cooling system and overall lamp performance.
Proper cooling ensures that lamps operate at optimal temperature and pressure for output power, spectrum, lamp
life, light guide life and safety.
4.1 Ensuring adequate cooling
Never obstruct the air vents on the X-Cite® unit. Vents are located at the rear and 1.
X-Cite® models vents were located on the side, underneath the lamp access panel.)
Do not remove the rubber feet on the X-Cite® unit or otherwise reduce/block the
through the unit.
Ensure that the air being used to ventilate the X-Cite® unit is approximately “room temperature 3.
(e.g. do not place the X-Cite® unit on top of another heat-producing instrument).
If a heated environmental chamber is being used for live cell imaging, make sure that the X-Cite® 4.
unit and the light guide are located outside of the chamber.
X-Cite® 120 Original Series X-Cite® 120XL or Q Series
Flllmescence lHllmlnauun - lrl Conucl X-Cite® Guide out wrth a can of compressed alr lf usmg compressed alr, dlrect the alrfluw ACROSS the vents, not 4.3 How to check/clean X-Cite“ 120 (original design) nylon foam filters able to see a grey mesh filter on the mslde orthe urut. Thls filter requlves cleanlng or replaemg lf. Dust/lmt/deorls IS blocklng the Vlew ofthe grey mesh filter The filter looks as though rt IS crumblmg lr IS posslble to (lean hewerfilters, but It IS generally better to replaCe them, as they tend to deterlorate Techrllcal Support for addrtlonal mrormatron on cleanlng/veplaclng filters 44 What happens if filters are not (lean? A complete filter blockage or alrflow obstruetlon generally results m an automatle lamp shutorr wrthm loel 5 A partlally dogged filter may allow enough alrfiow for eontmued operatlon, but not for optlmal caollng. In thls EKCELITAS TECHNOLOGIES”
2260 Argentia Road,
Mississauga, Ontario,
Telephone: +1 905 821-2600
Toll Free (USA and Canada): +1 800 668-8752
Facsimile: +1 905 821-2055
4.2 How to check/clean X-Cite
120XL/Q metal vents
1. When unit is OFF and lamp is COOL, unplug the power from the X-Cite® unit and turn the unit upside
down. Visually inspect the metal gratings for lint, dust and other debris.
2. Debris can be removed from the gratings using a small vacuum cleaner with a crevice tool, or blown
perpendicularly (i.e. into the X-Cite® unit).
1. Visually inspect the perforations on the side of the unit, under the lamp access door. You should be
Dust/lint/debris has accumulated
over time (one reason they stopped being used in X-Cite® units in 2006). Contact Lumen Dynamics
minutes of the unit being powered on due to lack of adequate cooling.
case, the result is usually a dramatic reduction in lamp life, e.g. 400 hours instead of 2500+ hours, it may also cause
bubbles to form in the light guide.
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